Coding

Part:BBa_K2107000

Designed by: Cody Fell   Group: iGEM16_Lubbock_TTU   (2016-10-19)


Platelet Derived Growth Factor-B Isoform 1 Serine Modified

This Biobrick codes for Platelet Derived Growth Factor-Beta Isoform 1, it is a growth factor that has been shown to modulate cell proliferation in angiogenesis. Additionally, PDGF-B is preceded by the periplasmic localization signal DsbA. When imaging this PDGF-B in western blot it has been shown to appear between 28 and 37Kda. To help with protein purification this part also includes a His tag at the c-terminus.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None